Sodium polyanetholesulfonate in the identification of Gardnerella vaginalis
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Sodium polyanetholesulfonate in the identification of Gardnerella vaginalis.
With respect to paragraph 6 of Dr. L0vborg's letter, the solid phase was blocked with BSA and Tween as described. The assessment of the plates did not occur in only a few experiments. We worked with the Nunc plates using the complete ELISA procedure as described for many months before evaluating other types of plates. Finally, the view held by Dr. L0vborg in paragraph 3 that detergent is a weak...
متن کاملEffect of sodium polyanetholesulfonate and gelatin on the recovery of Gardnerella vaginalis from blood culture media.
Sodium polyanetholesulfonate (SPS) is used as a routine supplement to blood culture media to enhance recovery of microorganisms, but it inhibits the growth of Peptostreptococcus anaerobius, Neisseria meningitidis, Neisseria gonorrhoeae, and Streptobacillus moniliformis. Comparative clinical blood culture studies at the University of Colorado Hospital suggested that SPS also inhibits the growth ...
متن کاملIdentification of Gardnerella (Haemophilus) vaginalis.
Different tests for the identification of Gardnerella (Haemophilus) vaginalis and for its differentiation from catalase-negative unclassified coryneforms from the vagina were evaluated on over 200 bacterial strains, with special emphasis on optimal test conditions. A presumptive identification of G. vaginalis in the clinical laboratory can be made on the basis of colonial morphology, clear beta...
متن کاملBiotypes of Gardnerella vaginalis.
A simple and reproducible scheme for identifying biotypes of Gardnerella vaginalis has been developed, based on reactions for lipase, hippurate hydrolysis, and beta-galactosidase. Among a total of 359 strains tested, eight biotypes were observed, the most common ones being types 1 (beta-galactosidase positive, lipase positive, hippurate positive), 2 (beta-galactosidase negative, lipase positive...
متن کاملIdentification of Gardnerella vaginalis with the API 20 Strep system.
A total of 137 strains of Gardnerella vaginalis were examined by the API 20 Strep system. The system was shown to be reliable when the tests were compared with standard identification methods, and very little confusion occurred with streptococcal profiles; consequently, G. vaginalis has been included in the API 20 Strep data base.
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1985
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.22.2.324-325.1985